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HeLa Extract
HeLa cytoplasmic extract. HeLa extract is required to show SV40 DNA replication in the presence of 1 µg SV40 Large T Antigen in vitro.
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Only %1 left
SKU
6030
Source
Cultured HeLa Cells
Function Definition
HeLa Extract is required to show SV40 DNA replication in the presence of 1 µg SV40 Large T Antigen in vitro (1)
Storage Buffer
20 mM Hepes (ph 7.5)
50 mM NaCl
1 mM dithiothreitol
0.1 mM EDTA
10% (v/v) glycerol
Assay Conditions
According to the method of Li and Kelly, as provided in the SV40 DNA Replication Assay, CHIMERx catalog number 8050 (1), (2), (3)
Storage Conditions
Store at -80ºC
Once thawed, aliquots of HeLa Extract that are not used should be discarded
Freeze-thaw cycles will result in diminished performance of the extract
Categories
Certificate of Analysis PDF -Current Lot
MSDS PDF - Current Lot
- SV40 DNA Replication Assay Kit, CHIMERx Catalog number 8050, Instruction Manual, Version 1.6
- Li, Joachim J. and Kelly, Thomas J. (1985) Mol Cell Biol 5, 1238-1246
- Stillman, B.W. and Gluzman, Y. (1985) Mol Cell Biol 5, 2051-2060
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