Cloned AMV Reverse Transcriptase

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot     

Reagents Supplied

5X AMV Reverse Transcriptase Reaction Buffer

Source

Avian myeloblastosis virus

Description

The only true clone derived from the Avian myeloblastosis virus, (AMV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template

• The enzyme is purified from a recombinant source
  
• Comparable activity to Native AMV Reverse Transcriptase
  
• Exhibits improved processivity compared to M-MLV, Native AMV-RT, and other clones generating longer cDNA products
• Maintains the RNA- and DNA-dependent DNA polymerase and RNase H activities
  
• RNase H activity can be regulated over a wide range of temperatures
  
• Expressed as a very stable, highly active polymer
  
• Remarkably robust in cDNA synthesis and RT-PCR

Applications

• Capable of synthesizing cDNA over a wide range of temperatures
• Ideal for use in RT-PCR, cDNA libraries, RAMP™, NASBA™ and dideoxy-DNA sequencing (1,2,3)

Unit Definition

One unit is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid insoluble material in 10 minutes at 37°C (4)

Concentration

20 - 30 units/µl

Assay Conditions

50 mM Tris HCl (pH 8.3)
10 mM MgCl₂
40 mM KCl
0.8 mM [³H] dTTP
0.2 mM poly(rA)·(dT)50
Reaction volume of 100 µl

Storage Buffer

200 mM potassium phosphate (pH 7.2)
5 mM dithiothreitol
0.2% Triton X-100™
50% glycerol

Storage Conditions

Store at -20°C
Products shipped on Dry Ice

References

(1) Goodman, H.M. and MacDonald, R.J. (1979) Methods Enzymol. 69, 75-90
(2) Naylor, L.H. and Van De Sande, J.H. (1986) Nucleic Acids Res. 14, 5939
(3) Zagursky, R.J., Baumeister, K., Lomaz, N. and Berman, M.L. (1985) Gene Anal. Tech. 2, 89-94
(4) Houts, G.E., Masakau, M., Ellis, C., Beard, D. and Beard, J.W. (1979) J. Virol. 29, 517-522