Ban II

Catalog No. 2060

Availability: In stock

$0.00

Quick Overview

5'...GPuGCPyC...3'
3'...CPyCGPuG...5' 

* Required Fields

$0.00

Details

Source

Bacillus aneurinolyticus

Reagents Supplied

CMX Buffer 1

Unit Definition 

One unit is the amount of enzyme required to completely digest 1μg of lambda DNA in 1 hour in a total reaction volume of 50 μl

Assay Conditions

10 mM Tris-HCl (7.5 pH at 37°C)
10 mM magnesium acetate
1 mM dithiothreitol
100 μg/mL bovine serum albumin
1 μg of lambda DNA
Incubation is at 37°C for 1 hour in a reaction volume of 50 μl

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)
20 mM NaCl
0.1 mM EDTA
1 mM dithiothreitol
200 ug/ml bovine serum albumin
0.01% Triton X-100™
50% (v/v) glycerol

Storage Conditions

Store at -20°C
Product shipped on Dry Ice