Cycle-Sequal™ DNA Polymerase

Catalog No. 8020

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Quick Overview

Stable thermophilic DNA polymerase suitable for applications requiring high temprature synthesis of DNA

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Details

Description

Cycle-Sequal™ DNA Polymerase is a stable thermophilic DNA polymerase suitable for applications requiring high temprature synthesis of DNA

Unit Definition

One unit is the amount of enzyme required to incorporate 10 nmoles of total nucleotide into acid-insoluble material in 30 minutes at 37°C

Assay Conditions

25 mM Tris-HCl (pH 9.5)
10 mM MgCl2
1.0 mg/ml of bovine serum albumin
50 mM KCl
0.2 mM of each dCTP, dGTP and dTTP
15 µg activated DNA

Storage Buffer

50 mM Tris HCl (pH 7.5)
5.0 mM DTT
0.1 mM EDTA 
50% (v/v) glycerol

Storage Conditions

Store at -20°C
Product shipped on dry ice