Mung Bean Nuclease

Catalog No. 1190

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Quick Overview

Single-stranded specific DNA and RNA endonuclease

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Details

Mung Bean Nuclease Source

Mung bean sprouts

Reagents Supplied

10X Mung Bean Nuclease Reaction Buffer

Applications

  • Removes protruding ends in duplex DNA resulting in ligatable blunt ends (1).
  • Suitable for trimming single-stranded protruding ends of DNA or RNA without degrading the duplex structure (2).
  • Used for mapping transcripts by analyzing the Mung Bean Nuclease-resistant RNA-DNA hybrids (3).
  • Digests hairpin structures during cDNA synthesis (4).
  • Will not cleave the strand opposite a nick in duplex DNA.
  • Requires Zn2+ ions for activity.

Unit Definition

One unit is the amount of enzyme required to produce 1 µg of acid-soluble material per minute at 37°C using denatured calf thymus DNA.

Reaction Buffer

30 mM NaOAc (pH 5.0 at 22°C)
50 mM NaCl
1 mM ZnCl2 
5% glycerol 

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 22°C)
0.1 mM zinc acetate
50% (v/v) glycerol
1 mM Cystein 

Assay Conditions 

30 mM sodium acetate (pH 5.0 at 22°C).
50 mM NaCl.
1 mM ZnCl2.
0.5 mg/ml denatured calf thymus DNA.
5% (v/v) glycerol.
Incubation at 37ºC for 10 minutes in a total reaction volume of 1 ml.

Quality Control

All preparations are assayed for contaminating double-stranded DNase activity.

References

(1) Ardelt, W. and Laskowski, M., Sr., Biochem. Biophys. Res. Commun. 44, No. 5, 1201-1211. 1971
(2) Berk, A.J. and Sharp, P.A., Cell 12, 721-732. 1977
(3) Berk, A.J. and Sharp, P.A., Proc. Natl. Acad. Sci. USA 75 1274-1278. 1978
(4) Goodman, H.M. and McDonald, R.J., Methods Enzymol. 68, 75-90. 1979 

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