Details
Description
- Ideal for sizing proteins on acrylamide gels under denaturing conditions
- Consists of 11 bands with fragments of the following sizes: 10, 15, 23, 25, 30, 40, 50, 75, 100, 150, and 200 kDA
- For easy reference, the protein bands at 25 and 75 kDA are three times (3X) as intense as any other bands in the ladder
- Supplied in ready-to-load buffer containing tracking dyes
Loading
For Coomasie Brilliant Blue R-250™ stained gels, the recommended amount of size marker to load on a gel is 5.0 µl per lane.
For Siver stained gels, dilute protein ladder 1:15 in 1X loading buffer and load 5 µl per lane.
Storage Conditions
Store at 4ºC Short Term
Store Long Term at -20°C
Notes: Product contains SDS, which may precipitate during storage
Mix well after thawing
If necessary, warm protein ladder at 37ºC until product clarifies
