E. coli DNA Ligase

Catalog No. 1065

Quick Overview

Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl cohesive termini in duplex DNA

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Details

Source

Escherichia coli

Reagents Supplied

5X E. coli Reaction Buffer

Applications

  • Catalyzes the formation of a phosphodiester bond between duplex DNA fragments with cohesive ends
  • Suitable for high-efficiency cloning of full-length cDNA (1,2)
  • Condensation of the 5'-phosphoryl group with adjacent 3'-hydroxyl group is coupled with the hydrolysis of NAD+

Unit Definition

One unit is the amount of enzyme required to yield 50% ligation of HindIII fragments of lambda DNA in 30 minutes at 16°C in a 20 µl assay mixture containing a DNA terminus concentration of 0.12 µM (300 µg/ml).

Storage Buffer

10 mM Tris-HCl (pH 7.4)
50 mM KCl
0.1 mM EDTA
10 mM ammonium sulfate
1 mM dithiothreitol 
50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below.

Storage Conditions

Store at -20°C
Product shipped on dry ice

Downloads

Certificate of Analysis
Lot 2205010 PDF 

MSDS
MSDS PDF

References

(1) Okayama, H. and Berg, P. (1982) Mol. Cell. Bio. 2, 161-170
(2) Gubler, U. and Hoffman, B.J. (1983) Gene 25, 263-269