Alkaline Phosphatase, Calf Intestinal

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot   

Reagents Supplied

10X CIP Reaction Buffer

Source

Calf intestinal mucosa

Applications

• Has higher turnover rate and milder conditions of inactivation than bacterial enzyme (1)
• Can be used to remove 5'-phosphates from DNA or RNA prior to 5'-end labeling (1)
• Used to remove 5'-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures (1)
• Applicable to dephosphorylation of proteins

Unit Definition

One unit is the amount of enzyme required to hydrolyze 1 µmol of p-nitrophenylphosphate to p-nitrophenol in 1 minute at 37°C (2)

Concentration

20 - 25 units/μl

Reaction Buffer

500 mM Tris-HCl (pH 9.0)
10 mM MgCl₂ 

Storage Buffer

10 mM Tris HCl (pH 8.0)
50 mM KCl
1 mM MgCl₂
0.1 mM ZnCl₂
50% (v/v) glycerol

Assay Conditions

1 M diethanolamine (pH 9.8)
10 mM p-nitrophenylphosphate 
0.25 mM MgCl₂

Storage Conditions

Store at -20°C
Product shipped on dry ice

References

(1) Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition, pp. 5.72, Cold Spring Harbor, New York, NY
(2) Mosser, E., Boll, M., and Pfleiderer, G. (1980) Hoppe-Seyler's Z. Physiol. Chem. 361, 543-549