S1 Nuclease

Source

Aspergillus oryzae

Applications

• Catalyzes degradation of single-stranded nucleic acids into oligonucleotides and 5'-mononucleotides (1)
• Cleaves both single-stranded DNA and RNA, with stronger DNAse activity
• Double-stranded DNA, double-stranded RNA and DNA-RNA hybrids are resistant to degradation at moderate enzyme concentration
• Capable of cleavage of double-stranded nucleic acids at nicks, mismatches and small gaps (2)
• Relaxes/linearizes supercoiled plasmids
• Removes protruding single-stranded ends
• Used for S1 mapping of nucleic acids
• Requires Zn²+ ions for activity
• The enzyme is active up to 65°C

Storage Buffer

20 mM Tris-HCl (pH 7.5 at 22°C)
50 mM NaCl
0.1 mM ZnCl₂
50% (v/v) glycerol

Assay Conditions

30 mM sodium acetate (pH 4.6)
1 mM zinc acetate
50 mM NaCl
0.5 mg/ml activated DNA
5% glycerol
Incubation at 37ºC for 10 minutes in a total reaction volume of 50 µl

Quality Control

All preparations are assayed for contaminating exonuclease and double-stranded DNase activities

Storage Conditions

Store at -20°C
Product shipped on Dry Ice 

References

(1) Berg, A.J. et al. (1977) Cell 12, 721
(2) Sambrook, J. et al. (1989) Molecular cloning: A laboratory Manual, second edition, pp. 5.78-5.79, Cold Spring Harbor, New York