S1 Nuclease

Catalog No. 1335

Quick Overview

Non-specific endonuclease used for single-stranded DNA and RNA digestion

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Details

Source

Aspergillus oryzae

Applications

  • Catalyzes degradation of single-stranded nucleic acids into oligonucleotides and 5'-mononucleotides (1)
  • Cleaves both single-stranded DNA and RNA, with stronger DNAse activity
  • Double-stranded DNA, double-stranded RNA and DNA-RNA hybrids are resistant to degradation at moderate enzyme concentration
  • Capable of cleavage of double-stranded nucleic acids at nicks, mismatches and small gaps (2)
  • Relaxes/linearizes supercoiled plasmids
  • Removes protruding single-stranded ends
  • Can generate double-stranded DNA breaks in response to DNA nicks abasic sites
  • Used for S1 mapping of nucleic acids
  • Requires Zn²+ ions for activity
  • The enzyme is active up to 65°C

Unit Definition

One unit is the amount of enzyme required to convert 1 µg of denatured calf thymus DNA to an acid soluble form in 1 minute at 37°C.

Storage Buffer

20 mM Sodium Acetate (pH 4.6)
50 mM NaCl
1 mM ZnCl2
50% (v/v) glycerol

Quality Control

All preparations are assayed for contaminating exonuclease and double-stranded DNase activities.

Storage Conditions

Store at -20°C
Product shipped on Dry Ice 

Downloads

Certificate of Analysis
Lot 3506011 PDF 

References

(1) Berg, A.J. et al. (1977) Cell 12, 721
(2) Sambrook, J. et al. (1989) Molecular cloning: A laboratory Manual, second edition, pp. 5.78-5.79, Cold Spring Harbor, New York