Single-Stranded DNA Binding Protein, E coli

Source

Escherichia coli

Description

• Single-stranded specific DNA binding protein (1)
• Helix destabilizing protein (1)
• Ultrapure recombinant protein

Applications

• Reduces formation of secondary DNA structures
• Prevents degradation of single stranded DNA by nucleases
• Prevents inhibition of PCR by template DNA contaminants (2)
• Improves efficiency of DNA amplification by Taq DNA Polymerase (3,4,5,6)
• Replaces Hot Start method by assembling PCR reactions (4,7)
• Stabilizes single-stranded regions of DNA for site-specific mutagenesis
• Aids completion of restriction enzyme digestion
• Improves efficiency of DNA synthesis by T4 DNA Polymerase (8)
• Enhances fidelity of modified T4 DNA Polymerase (8)

Storage Buffer

20 mM Tris-HCl (pH 7.8 at 22°C)
300 mM NaCl
5 mM β-mercaptoethanol
0.05% Igepal
0.2 mM EDTA
50% (v/v) glycerol 

Quality Control

All preperations are assayed for contamination by endonuclease and 3'- and 5'-exonuclease activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis

Storage Conditions

Store at -20°C
Product shipped on Dry Ice

References

(1) Greipel, J. Urbanke, C. and Maass, G. (1989) in: Saenger, W., Heinemann, U. (Eds.) pp. 61-86
(2) Kreader, C.A. (1996) Applied Environ. Micro. 62, 1102-1106
(3) Dąbrowski, S., Olszewski, M., Piątek, R. and Kur, J. (2002) Protein Expr. Purif. 26, 131-138
(4) Dąbrowski, S. and Kur, J. (1999) Protein Expr. Purif. 16, 96-102
(5) Rapley, Mol. Biotech. 2 (1994) 295-298
(6) Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1989) Nucleic Acids Res. 18, 1079
(7) Barski, P., Piechowicz, L., Galinski, J. and Kur, J. (1996) Mol. Cell Probes 10, 471-475