RecA Protein

Catalog No. 1370

Availability: In stock

Price From: $67.00

Quick Overview

Bacterial recombination protein that enhances interaction and exchange of homologous DNA strands

* Required Fields

Price From: $67.00

Details

Description

  • A 37,8 kDa protein encoded by the recA gene of E coli.
  • Involved in DNA renaturation and rare cleavage of genomic DNA.
  • Ultrapure recombinant protein.

Applications

  • Regulates repair mechanisms and repairs DNA (1, 2).
  • Binds cooperatively and stechiometrically to single-stranded DNA.
  • In presence of ATP-g-S, locates and pairs a single-stranded DNA sequence to its homologous double-stranded DNA.
  • Cleaves large fragments of DNA (RARE-Rec A Assisted Restriction Endonuclease) (3).
  • Enhances contrast of electron micrography when DNA is coated with Rec A Protein (4).

Storage Buffer

20 mM Tris-HCl (pH 7.5 at 22°C)
1 mM dithiothreitol
0.1 mM EDTA  
50% (v/v) glycerol

Quality Control

All preparations are assayed for endonuclease and nonspecific single- and double-stranded DNase activities. Typically preparations are greater than 95% pure as judged by SDS polyacrylamide gel electrophoresis.

Storage Conditions

Store at -20°C
Shipped on Dry Ice 

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot      

References

(1) Clark, A.J. and Margulies, A.D. (1965) Proc. Natl. Acad. Sci. U.S.A. 53, 451-459
(2) Radding, C.M. (1978) Annu. Rev. Biochem. 47, 847-880
(3) Koob, M., Burkiewicz, A., Kur, J. and Szybalski, W. (1992) Nucleic Acids Res. 20, 5831-5836
(4) Krasnow, M. A. et al. (1983) Nature 304, 559