T4 DNA Ligase

Catalog No. 1060

Availability: In stock

Price From: $40.00

Quick Overview

Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA

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Price From: $40.00

Details

Source

T4 bacteriophage of Escherichia coli

Reagents Supplied

5X T4 DNA Ligase Buffer

Applications

  • Catalyzes the joining of duplex DNA molecules at blunt ends (1).
  • Covalently joins DNA fragments with complementary cohesive ends.
  • Seals single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
  • Suitable for cloning of restriction fragments and joining linkers or adapters to blunt-ended DNA (2).
  • Ultrapure recombinant enzyme.

Unit Definition

One (Weiss) unit is the amount of enzyme required to convert 1 nmol of 32P from pyrophosphate into Norit-adsorbable form in 20 minutes at 37°C.
Note: 67 cohesive end ligation units are the equivalent of one Weiss unit.

Heat Inactivation

65ºC for 15 minutes

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 22°C)
50 mM KCl
1 mM dithiothreitol
50% (v/v) glycerol

Reaction Conditions

See Certificate of Analysis Below

Storage Conditions

Store at -20°C
Product shipped on dry ice

Downloads

Certificate of Analysis
Lot 2010013 PDF

MSDS
MSDS PDF 

References

(1) Weiss, B., Jacquemin-Sablan, A., Live, T.R., Fareed, G.C. and Richardson, C.C. (1968) J. Biol. Chem. 243, 4543-4555
(2) Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition, 1.53-1.73