T4 Gene 32 Protein

Catalog No. 1170

Quick Overview

Single-stranded specific DNA binding protein

* Required Fields

Price From: $60.00

Details

Description

  • Single-stranded specific DNA binding protein (1)
  • Helix destabilizing protein (1)
  • Ultrapure recombinant protein

Applications

  • Reduces formation of secondary DNA structures
  • Prevents degradation of ssDNA by nucleases
  • Prevents inhibition of PCR by template NDA contaminants (2)
  • Replaces Hot Start method during assembling PCR reaction (4,7)
  • Improves the efficiency of DNA amplification by Taq Polymerase (3,4,5,6)
  • Enchances the fidelity of modified T4 Polymerase (8)
  • Stabilizes single-stranded regions of DNA for site-specific mutagenesis
  • Aids completion of restriction enzyme digestion
  • Improves the efficiency of DNA synthesis by T4 DNA Polymerase (8)

Storage Buffer

20 mM Tris-HCl (pH 8.0 at 22°C)
100 mM NaCl
1 mM dithiothreitol
1 mM EDTA
100 mM NaCl
50% (v/v) glycerol 

Quality Control

All preparations are assayed for contaminating endonuclease, 3'- and 5'-exonulease activities 
Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis

Storage Conditions

Store at -20°C
Shipped on Dry Ice

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF

References

1. Greipel, J. Urbanke, C. and Maass, G. (1989) in: Saenger, W., Heinemann, U. (Eds.) pp.61-86
2. Kreader, C.A. (1996) Applied Environ. Micro. 62, 1102-1106
3. Dabrowski, S., Olszewski, M., Piatek, R. and Kur, J. (2002) Protein Expr. Purif. 26, 131-138
4. Dabrowski, S. and Kur, J. (1999)Protein Expr. Purif. 16, 96-102
5. Rapley, Mol. Biotech. 2 (1994) 295-298
6. Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1989) Nucleic Acids Res. 18, 1079
7. Barski, P., Piechowicz, L., Galinski, K. and Kur, J. (1996) Mol. Cell Probes 10, 471-475
8. Sandhu, D.K. and Keohavong, P. (1994) Gene 144, 53-58