Taq Single-Stranded DNA Binding Protein

Source

Thermus aquaticus 

Description

• Thermostable single-stranded specific DNA binding protein (1)
• Helix destabilizing protein (1)
• Ultrapure recombinant protein

Applications

• Reduces formation of problematic secondary DNA structures
• Prevents degradation of ssDNA by nucleases
• Prevents inhibition of PCR by template DNA contaminants (2)
• Improves the efficiency of DNA amplification by Taq DNA Polymerase (3,4,5,6)
• Improves the specificity and selectivity of multiplex PCR (7)
• Aids PCR of difficult and GC-rich templates
• Stabilizes single-stranded regions of DNA for site-specific mutagenesis
• Aids completion of restriction enzyme digestion

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 22°C)
50 mM NaCl
5 mM β-mercaptoethanol
0.05% Igepal
0.1 mM EDTA
50% (v/v) glycerol

Quality Control

All preparations are assayed for contaminating endonuclease, 3'- and 5'-exonuclease activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis

Storage Conditions

Store at -20ºC
Product Shipped on Dry Ice 

References

(1) Greipel, J. Urbanke, C. and Maass, G. (1989) in: Saenger, W., Heinemann, U. (Eds.) pp. 61-86
(2) Kreader, C.A. (1996) Applied Environ. Micro. 62, 1102-1106
(3) Dąbrowski, S., Olszewski, M., Piątek, R. and Kur, J. (2002) Protein Expr. Purif. 26, 131-138
(4) Dąbrowski, S. and Kur, J. (1999) Protein Expr. Purif. 16, 96-102
(5) Rapley, Mol. Biotech. 2 (1994) 295-298
(6) Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1989) Nucleic Acids Res. 18, 1079
(7) Barski, P., Piechowicz, L., Galinski, J. and Kur, J. (1996) Mol. Cell Probes 10, 471-475