AMV Reverse Transcriptase

Downloads

Certificate of Analysis -Current Lot
MSDS PDF -Current Lot     

Reagents Supplied

5X AMV Reverse Transcriptase Reaction Buffer

Source

Avian Myeloblastosis Virus

Description

• Contains both reverse transcriptase and RNase H activities
• Maintains RNase H activity necessary for cDNA synthesis and isothermal RNA amplification

Applications

• Can be used for preparing labeled hybridization probes
• Ideal for use in RT-PCR, RAMP™, NASBA™, cDNA libraries and dideoxy-DNA sequencing (1, 2, 3)

Unit Definition

One unit is the amount of enzyme required to incorporate 1.0 nmol of labeled dTTP into acid insoluble material in 10 minutes at 37°C (4)

Concentration

20 - 30 units/µl

Storage Buffer

0.2 M potassium phosphate (pH 7.2) 
2 mM dithiothreitol
0.2% Triton X-100™
50% (v/v) glycerol

Assay Conditions

50 mM Tris HCl (pH 8.3)
10 mM MgCl₂
40 mM KCl
0.5 mM [³H] dTTP
0.4 mM poly(rA)·(dT)50
Reaction volume of 100 µl

Storage Conditions

Store at -20°C
Product shipped on Dry Ice

References

(1) Goodman, H.M. and MacDonald, R.J. (1979) Methods Enzymol. 69, 75-90.
(2) Naylor, L.H. and van de Sande, J.H. (1986) Nucleic Acids Res. 14, 5939
(3) Zagursky, R.J., Baumeister, K., Lomax, N. and Berman, M.L. (1985) Gene Anal. Techn. 2, 89-94
(4) Houts, G.E., Masakau, M., Ellis, C., Beard, D. and Beard, J.W. (1979) J. Virol. 29, 517-522