Plasmid Miniprep DNA Purification Kit

Catalog No. 3500

Availability: In stock

Price From: $70.00

Quick Overview

Kit designed to isolate very high purity plasmid DNA from various species of bacteria, including recombinant Escherichia coli strains

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Price From: $70.00

Details

Description

CHIMERx’s Plasmid Miniprep DNA Purification Kit is designed to isolate very high purity plasmid DNA from various species of bacteria, including recombinant Escherichia coli strains. Plasmid DNA contaminants such as: RNA, single-stranded DNA, enzymes/proteins, lipids, endotoxins, dyes, detergents, nucleotides, EDTA, problematic restriction and ligation inhibitors, buffers and salts are effectively removed from crude bacterial lysate. The resin version used for construction of the Plasmid Miniprep Kit is especially optimized toward removal of problematic inhibitors of restriction of DNA as well as non-specific endo- and exonucleases. Colored lysis buffer helps both in monitoring cell solubilization progress as well as simultaneous processing of multiple samples. Optimized buffer is added to provide selective conditions for DNA binding to the membrane during brief centrifugation, while contaminants pass through the spin-column. Traces of contaminants remaining on the membrane are efficiently removed in two wash steps. High-quality DNA is then eluted in low salt buffer, e.g.: Tris-HCl, TE or water. Isolated DNA is ready for downstream applications without the need for ethanol precipitation.

Regulatory Statement

For laboratory research use only. Not for use on humans or animals.

Storage Conditions

Store at 4°C
Shipped at room temperature

Downloads

Certificate of Analysis PDF - Current Lot
MSDS PDF - Current Lot 

Categories

Nucleic Acid Purification Kits