RNase I

Catalog No. 1300

Quick Overview

Completely nonspecific ribonuclease that hydrolyzes the phosphodiester bond of all four bases

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Price From: $42.00

Details

Description

  • Completely nonspecific hydrolyzing after all four bases (1)
  • Only available RNase that cleaves the phosphodiester bond of all four bases
  • Prefers single-stranded RNA to double-stranded RNA
  • Produced from an overexpressing clone in E. coli (2)
  • RNase I contains no endonuclease or exonuclease activity toward DNA substrates
  • No need for boiling prior to use
  • Pure, contains no endonuclease or exonuclease activity toward DNA substrates

Applications

  • RNase I degrades RNA to cyclic nucleotide monophosphates leaving a 5'-OH and 2'-, 3'-cyclic monophosphate
  • Ideal for ribonuclease protection assays
  • Useful for mapping or quantitation of RNA by selective cleavage of single-strand regions

Reagents Supplied

10X RNase I Reaction Buffer

Unit Definition

One unit is the amount of enzymes required to degrade 1 µg of RNA in 30 minutes at 37°C as detected by TCA precipitation

Storage Buffer

10 mM Tris-HCl (pH 8.0)
200 mM NaCl
50% glycerol

Assay Conditions

10 mM Tris-HCl (pH 8.0)
100 mM NaCl 

Quality Control

All preparations are assayed for contaminating exonuclease and nonspecific endonuclease activities

Storage Conditions

Store at -20°C
Shipped on Dry Ice

Downloads

MSDS PDF

References

(1) Meador, J. III, and Kennell, D. (1990) Gene 95, 1-7
(2) Meador, J. et al. (1990) Eur. J. Biochem. 187, 549