Pfu DNA Polymerase

Catalog No. 1114

Availability: In stock

Price From: $55.00

Quick Overview

Extremely thermostable proofreading DNA polymerase, suitable for applications requiring high temperature synthesis of DNA.

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Price From: $55.00

Details

Description

  • Pfu DNA Polymeraseis a thermostable enzyme isolated from hyperthermophilic archaea Pyroccocus sp. (1).
  • Ultrapure recombinant enzyme.
  • Unmodified enzyme replicates DNA at 74°C and exhibits over 95% activity after 1-hour incubation at 95°C.
  • The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´->3´ direction in the presence of magnesium ions.
  • The enzyme exhibits the 3´->5´ proofreading activity, resulting in over 10-fold higher PCR fidelity than possible with Taq DNA Polymerases (2).
  • Pfu DNA Polymerase is recommended for use in high-fidelity PCR, PCR of GC-rich sequences or problematic secondary structures and cloning of blunt-ended PCR products.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 min at 74°C.

Reaction Conditions

50 mM Tris-HCl (pH 9.0 at 25°C)
50 mM NaCl, 5 mM MgCl2
200 μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP)
10 μg activated calf thymus DNA
0.1 mg/ml BSA
Final volume 50 μl

Quality Control

All preparations are assayed for contaminating endonuclease, 3'-exonuclease, and nonspecific single- and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

References

  1. Lundberg, K., Shoemaker, D., Adams, M., Short, J., Sorge, J. and Mathur E. (1991) Gene 108, 1.
  2. Cline, J., Braham, J. and Hogrefe, H. (1996) Nucleic Acids Res. 24, 3546.