Taq DNA Polymerase

Catalog No. 1111

Availability: In stock

Price From: $70.00

Quick Overview

Stable Recombinant thermophilic DNA polymerase, suitable for applications requiring high temperature synthesis of DNA

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Price From: $70.00



Thermus aquaticus


  • Thermostable enzyme of approximately 94 kDa from Thermus aquaticus
  • Ultrapure, Recombinant protein


  • Recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 Kb
  • Taq DNA Polymerase enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C (1, 2)
  • Taq DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium ions
  • Maintains the 5'→3' exonuclease activity
  • Lacks the 3'→5' exonuclease activity

Reagents Supplied

10X Taq Buffer A
10X Taq Buffer B
10X Taq Buffer C 

Unit Definition 

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C

10X Reaction Buffer

10X Taq Buffer A (optimization buffer without MgCl2): Buffer allows to optimize MgClconcentration
10X Taq Buffer B (general application, up to 10 kb): Buffer contains 15 mM MgCland is optimized for use with 0.2 mM of each dNTP
10X Taq Buffer C (colored): 10X Taq Buffer B Enriched with two gel tracking dyes and a gel loading reagent; Enables direct loading of PCR products onto an agarose gel

Storage Buffer

20 mM Tris-HCl (pH 8.0 at 22°C)
100 mM KCl
0.1 mM EDTA
1 mM dithiothreitol
50% glycerol 

Assay Conditions

25 mM Tris-HCl (pH 9.5 at 25°C)
50 mM KCl
10 mM MgCl2
1 mM dithiothreitol
200 μM each of dCTP, dGTP, dTTP, and dATP (a mix of unlabeled and [α-32P] dATP)
10 μg activated calf thymus DNA 
1 mg/ml bovine serum albumin
15 µg activated calf thymus DNA
Total reaction volume is 50 µl

Quality Control

All preparations are assayed for contaminating endonuclease, 3'-exonuclease, and nonspecific single- and double-stranded DNase activities
Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis

Storage Conditions

Store at -20°C
Shipped on dry ice 




1. Chien, A., Edgar, D.B. and Trela, J.M. (1976) J. Bacteriol. 127, 1550
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980) Biokhimiya 45, 644