Bst DNA Polymerase, Large Fragment

Catalog No. 1078

Quick Overview

Large exonuclease free fragment of thermophilic Bst DNA Polymerase with strand displacement activity

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Price From: $42.00

Details

Source

Bacillus stearothermophilus

Description

  • Moderately thermostable enzyme from Bacillus stearothermophilus
  • Bst DNA Polymerase, Large Fragment exhibits thermophilic reverse transcriptase activity
  • Active over a wide range of reaction buffer conditions and magnesium ion concentrations
  • Ultrapure recombinant protein

Applications

  • Ideal for DNA synthesis reactions requiring strand displacement
  • Used in isothermal nucleic acids amplification
  • Used in isothermal DNA sequencing at elevated temperatures
  • Ccatalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium ions
  • Lacks the 5'→3' exonuclease activity, while retaining the polymerase activity (1)
  • Broad activity range; can replace mesophilic polymerases as well as synthesize DNA at high temperatures, therefore suitable for amplification of difficult DNA templates, including repetitive sequences, GC-rich regions and problematic secondary structures (2, 3)
  • Can be heat inactivated at temperatures above 80°C
  • Replicates DNA optimally at 65°C

Unit Definition

One unit is the amount of enzyme required to incorporate 10 nmoles of total deoxyribonucleotide into acid-isoluble form in 30 minutes at 60°C.

Storage Buffer

20 mM potassium phosphate (pH 7.1)
1.0 mM dithiothreitol
50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 8.6 at 22°C)
100 mM KCl
10 mM MgCl2
1.0 mg/ml bovine serum albumin
100 mM KCl
1.0 mM dithiothreitol
0.2 mM of each dCTP, dGTP, dTTP, and [α-32P]dATP
15 μg activated DNA
Incubation at 60°C for 30 minutes
Reaction volume of 50 μl

Quality Control

All preparations are assayed for contaminating endonuclease, exonuclease, and single- and double-stranded DNase activities

Storage Conditions

Store at -20ºC
Product shipped on dry ice 

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF

References

1. Stenesh, J. and Roe, B.A. (1972) Biochim. Biophys. Acta. 272, 156-166
2. Hugh, G. and Griffin, M. (1994) PCR Technology, p.p. 228-229
3. McClary, J. et al. (1991) J. DNA Sequencing and Mapping, p.p. 173-180