Human DNA Polymerase Alpha

Catalog No. 1075

Availability: In stock

Price From: $300.00

Quick Overview

Exclusively from CHIMERx, Human DNA Polymerase Alpha is a replication enzyme used to test the toxicity of drugs on key human replication systems

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Price From: $300.00

Details

Description

  • The simplest DNA Polymerase known in both size and catalysis
  • Replication enzyme involved in initiation of DNA replication in eukaryotes
  • A multi-subunit complex, which inhibits DNA polymerase and primase activities
  • Isolated from HeLa cells by immunoaffinity chromatography (1)

Applications

  • Used to test in vitro replication capabilities along with other enzymes
  • Used to test the toxicity of drugs on key human replication systems
  • Used in DNA replication experiments

Unit Definition

One unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 minutes at 37°C

Storage Buffer

20 mM Tris-HCl (pH 8.0)
0.25 mM EDTA
50 mM NaCl
1 mM β-mercaptoethanol
0.1% Triton X-100™
50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below.

Storage Conditions

Store at -80°C long term
Store at -20°C short term
Shipped on dry ice

Downloads

Certificates of Analysis

Lot 2404003 PDF 

MSDS 
MSDS PDF

References

(1) Podust, V.N., Lavrik, O.I., Nasheuer, H.P., and Grosse, F. (1989) FEBS Letters 245, 14-16