Human DNA Polymerase Alpha

Catalog No. 1075

Availability: In stock

Price From: $300.00

Quick Overview

Exclusively from CHIMERx, Human DNA Polymerase Alpha is a replication enzyme used to test the toxicity of drugs on key human replication systems

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Price From: $300.00



  • The simplest DNA Polymerase known in both size and catalysis
  • Replication enzyme involved in initiation of DNA replication in eukaryotes
  • A multi-subunit complex, which inhibits DNA polymerase and primase activities
  • Isolated from HeLa cells by immunoaffinity chromatography (1)


  • Used to test in vitro replication capabilities along with other enzymes
  • Used to test the toxicity of drugs on key human replication systems
  • Used in DNA replication experiments

Unit Definition

One unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 minutes at 37°C

Storage Buffer

20 mM Tris-HCl (pH 8.0)
0.25 mM EDTA
50 mM NaCl
1 mM β-mercaptoethanol
0.1% Triton X-100™
50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below.

Storage Conditions

Store at -80°C long term
Store at -20°C short term
Shipped on dry ice


Certificates of Analysis

Lot 2404003 PDF 



(1) Podust, V.N., Lavrik, O.I., Nasheuer, H.P., and Grosse, F. (1989) FEBS Letters 245, 14-16