Human DNA Polymerase Beta

Catalog No. 1077

Availability: In stock

Price From: $210.00

Quick Overview

Exclusively from CHIMERx, Human DNA Polymerase Beta is a repair enzyme used to fill gaps and nicks in double-stranded DNA

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Price From: $210.00

Details

Description

  • Simplest DNA polymerase known in both size and catalysis (1)

Applications

  • Repair polymerase able to synthesize DNA beyond the end of a gap or nick with simultaneous displacement of the non-replicated strand (2)
  • Fills gaps and nicks (3)

Unit Definition

One unit is the amount of enzyme required to incorporate 1 pmole of total nucleotide into acid-insoluble form in 60 minutes at 37°C under the stated assay conditions

Storage Buffer

20 mM Tris-HCl (pH 8.0)
0.1 mM EDTA
200 mM NaCl
1 mM dithiothreitol
50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 8.7)
100 mM KCl
10 mM MgCl2
0.4 mg/ml bovine serum albumin
1.0 mM dithiothreitol
15% (v/v) glycerol
0.05 mM of each dCTP, dGTP, dTTP, and [α-32P]dATP
10 μg activated DNA
Reaction volume of 50 μl

Quality Control

All preparations are assayed for contaminating endonuclease and exonuclease activities

Storage Conditions

Store at -80°C Long Term
Store at -20°C
Product shipped on dry ice

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF  

References

1. Abbotts, J., SenGupta, D.N., Zmudzka, B., Widen, S.G., Notario, V., and Wilson, S.H. (1988) Biochemistry 27, No. 3, 901-909
2. Nowak, R., Kulik, J., and Siedlecki, J.A. (1987) Acta Biochim Pol. 34, 205-215
3. Wang, T.S.F., and Korn, D. (1980) Biochemistry 19, 1782-1790