Details
Downloads
Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot
Description
- The simplest DNA Polymerase known in both size and catalysis
- Replication enzyme involved in initiation of DNA replication in eukaryotes
- A multi-subunit complex, which inhibits DNA polymerase and primase activities.
- Isolated from HeLa cells by immunoaffinity chromatography (1)
Applications
- Used to test in vitro replication capabilities along with other enzymes
- Used to test the toxicity of drugs on key human replication systems
- Used in DNA replication experiments
Unit Definition
One unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 minutes at 37°C
Storage Buffer
20 mM Tris-HCl (pH 8.0)
0.25 mM EDTA
50 mM NaCl
1 mM β-mercaptoethanol
0.1% Triton X-100™
50% (v/v) glycerol
Assay Conditions
60 mM Tris-HCl (pH 8.0)
5.0 mM magnesium acetate
0.3 mg/ml bovine serum albumin
1.0 mM dithiothreitol
0.1 mM spermine
0.2 mM of each dCTP, dGTP, dTTP, dATP (pH 7.0)
0.1 mM [α-3H]dATP
20 μg activated calf thymus DNA
Incubation at 37ºC for 30 minutes in a total reaction volume of 30 μl
Quality Control
Polymerase is tested for significant polymerase as primase activity. All preparations are assayed for contaminating endonuclease, 3'- and 5'-exonuclease, nonspecific RNase, and single- and double-stranded DNase activities.
Storage Conditions
Store at -80°C long term
Store at -20°C short term
Product shipped on dry ice
References
(1) Podust, V.N., Lavrik, O.I., Nasheuer, H.P., and Grosse, F. (1989) FEBS Letters 245, 14-16
Helpful Links
Serving the life sciences industry since 1992, Chimerx has earned the reputation as a leading manufacturer of the highest quality biochemical reagents available.
You have no items in your shopping cart.
You have no items to compare.