HIV Reverse Transcriptase

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Description

• Human Immunodeficiency Virus (HIV) Reverse Transcriptase is an RNA directed DNA polymerase which can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template

Applications

• Provides an excellent target for evaluating antiviral agents or inhibitors (1,2)
• Catalyzes error-prone synthesis on DNA and RNA templates - ideal for introducing random mutations (3)

Unit Definition

One unit is the amount of enzyme required to incorporate 1 nmol of labeled dTTP into acid insoluble material in 10 minutes
at 37°C

Concentration

10 - 30 units/µl

Assay Conditions

50 mM Tris HCl (pH 8.3)
6 mM MgCl₂
40 mM KCl
0.5 mM [³H] TTP
0.4 mM polyA(dT)_18-20
Reaction volume of 50 µl

Storage Buffer

20 mM potassium phosphate (pH 7.1)
1.0 mM dithiothreitol
0.02% Triton X-100™
50% glycerol

Storage Conditions

Store at -20°C
Product shipped on Dry Ice

References

(1) Hirsch, M. S., Kaplan, J. C. (1985) Ann. Intern. Med. 103, 750-755
(2) Tisdale, M. et al (1989) J. Antimicrob. Chemother. 23, 47-54
(3) Williams, K. J., Loeb, L. A. (1992) Curr. Top. Microbiol. Immunol. 176, 80-165