Thermus Flavus (Tfl) DNA Polymerase

Catalog No. 1112

Availability: In stock

Price From: $380.00

Quick Overview

Stable thermophilic DNA polymerase, suitable for applications requiring high temperature synthesis of DNA

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Price From: $380.00

Details

Source

Thermus flavus

Applications

  • Efficiently synthesizes DNA at elevated temperatures (1)
  • Can be used for amplification of nucleic acids in PCR and LCR reaction
  • Used for high temperature DNA sequencing
  • Broader Mg2+ ions concentration tolerance than Taq DNA polymerase

Reagents Supplied

10X Tfl DNA Polymerase Reaction Buffer

Unit Definition

One unit is the amount of enzyme required to incorporate 10 nmoles total nucleotide into acid insoluble material at 70°C for 30 minutes

Concentration

3.0 units/µl

Storage Buffer

50 mM Tris HCl (pH 7.5) 
0.1 mM EDTA
5 mM dithiothreitol
50% (v/v) glycerol 
Stabilizers

Assay Conditions

25 mM Tris-HCl (pH 9.5 at 25°C)
50 mM potassium chloride
10 mM MgCl2
1 mM dithiothreitol
1 mg/ml bovine serum albumin
300 µg/ml activated calf thymus DNA
0.2 mM of each dCTP, dGTP, dTTP and [α-32P]dATP
Incubation is for 10 minutes at 70°C in a reaction volume of 50 µl

Storage Conditions

Store at -20°C
Product shipped on dry ice

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF 

References

1. Kaledin, A. S., Sliusarenko, A.G. and Gorodetskii, S. I. (1980) Biokhimiya 45, 644-651