Thermus Flavus (Tfl) DNA Polymerase

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot     

Reagents Supplied

10X Tfl DNA Polymerase Reaction Buffer

Source

Thermus flavus

Applications

• Efficiently synthesizes DNA at elevated temperatures (1)
• Used for high temperature DNA sequencing
• Broader Mg2+ ions concentration tolerance than taq DNA polymerase
• Can be used for amplification of nucleic acids in PCR and LCR reaction

Unit Definition

One unit is the amount of enzyme required to incorporate 10 nmoles total nucleotide into acid insoluble material at 70°C for 30 minutes

Concentration

3.0 units/µl

Storage Buffer

50 mM Tris HCl (pH 7.5) 
0.1 mM EDTA
5 mM dithiothreitol
50% (v/v) glycerol 
Stabilizers

Assay Conditions

25 mM Tris-HCl (pH 9.5 at 25°C)
50 mM potassium chloride
10 mM MgCl₂
1 mM dithiothreitol
1 mg/ml bovine serum albumin
300 µg/ml activated calf thymus DNA
0.2 mM of each dCTP, dGTP, dTTP and [α-³²P]dATP
Incubation is for 10 minutes at 70°C in a reaction volume of 50 µl

Storage Conditions

Store at -20°C
Product shipped on dry ice

References

(1) Kaledin, A. S., Sliusarenko, A.G. and Gorodetskii, S. I. (1980) Biokhimiya 45, 644-651