Tth DNA Polymerase

Catalog No. 1115

Availability: In stock

Price From: $340.00

Quick Overview

Minimizes problems with strong secondary structure of RNA

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Price From: $340.00

Details

Reagents Supplied

10X Tth DNA Polymerase Reaction Buffer

Source

Thermus thermophilus

Applications

  • Ccan reverse transcribe at elevated temperatures
  • Minimizes problems with strong secondary structure of RNA
  • Results in greater specificity of primer hybridization and extension
  • Suitable for high temperature synthesis of DNA
  • Synthesizes cDNA from RNA template
  • Stable thermophilic DNA polymerase (1)

Unit Definition

One unit is the amount of enzyme required to incorporate 10 nmoles total nucleotide into acid insoluble material at 70°C for 30 minutes.

Concentration

5 - 10 units/µl

Storage Buffer

50 mM Tris HCl (pH 7.5) 
0.1 mM EDTA
5.0 mM dithiothreitol
50% (v/v) glycerol 
Stabilizers

Assay Conditions

25 mM Tris-HCl (pH 9.5 at 25°C)
50 mM potassium chloride
10 mM magnesium chloride
1 mM dithiothreitol
0.3 µg/μl activated calf thymus DNA
1 μg/μl  bovine serum albumin
0.2 mM of each dCTP, dGTP, dTTP and [α-32P]dATP
Incubation is for 10 minutes at 70°C in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Product shipped on dry ice

References

(1) Wang, A.M., Doyle, M.V., and Mark, D.F. (1989) Proc. Nat’l. Acad. Sci. USA 86, 9717-9721

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot   

Categories

Polymerases, Mesophilic DNA Polymerases