Acc I

Catalog No. 2000

Quick Overview

5'...GT↓MKAC ...3'
3'...CAKM↑TG...5' 

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Price From: $65.00

Details

Source

Acinetobacter calcoaceticus

Reagents Supplied

CMX Buffer 4

Unit Definition 

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 80°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-acetate (pH 7.5 at 37°C)
10 mM magnesium acetate
50 mM potassium acetate
1 mM dithiothreitol
100 μg/mL bovine serum albumin

Activity in Buffers

Buffer 1: <25%
Buffer 2: >75%
Buffer 3: 25%
Buffer 4: 100%
Buffer 5: 100%

Assay Conditions

See Certificate of Analysis Below

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)
10 mM BME
0.1 mM EDTA
0.5 mg/ml bovine serum albumin
0.1% Triton X-100™
50% (v/v) glycerol

Storage Conditions

Store at -20°C
Shipped on Dry Ice 

Downloads

Certificates of Analysis
Lot 2404005 PDF 
Lot 2309003 PDF 

MSDS
MSDS PDF

 

Unit Definition
Unit Definition