Acv I

Catalog No. 2005

Quick Overview

5'...CAC↓GTG...3'
3'...GTG↑CAC...5'

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Price From: $60.00

Details

Source

Aeromonas caviae

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA/Hind III fragments in 1 hour in a total reaction volume of 50 µl

Star Activity

No

Incubation Temperature

37°C

Heat Inactivation

80° for 10 minutes

Assay Unit Substrate

Ad-2 DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-Acetate (pH 7.5 at 37°C)
10 mM magnesium acetate
50 mM potassium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 75%
Buffer 2: 100%
Buffer 3: 50%
Buffer 4: 100%
Buffer 5: 75%

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 4°C) 
1 mM dithiothreitol
50 mM KCl
0.1 mM EDTA
10 mM MgCl2
200 µg/ml bovine serum albumin  
50% (v/v) glycerol

Assay Conditions

20 mM Tris-Acetate (pH 7.5 at 37°C)
10 mM Mg Acetate
50 mM K Acetate
1 mM dithiothreitol 
100 µg/ml bovine serum albumin  
1 µg Ad-2 DNA

Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on Dry Ice

Downloads

Certificates of Analysis
Lot 2404039 PDF

MSDS
MSDS PDF