BamH I

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Source

Bacillus amyloliquefaciens H

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour at 37°C in a reaction volume of 50 μl

Incubation Temperature

37°C

Heat Inactivation

No

Reaction Buffer

50 mM Tris-HCl (pH 7.5 at 22°C)
10 mM MgCl₂
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Assay Conditions

50 mM Tris‑HCl (pH 7.5 at 37°C)
10 mM MgCl₂
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 μg lambda DNA
Incubation for 1 hour at 37°C in a reaction volume 50 µl

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)
300mM KCl
0.5 mM EGTA
1 mM DTT
500 μg/ml bovine serum albumin
0.1% Triton X-100™
50% (v/v) glycerol

Storage Conditions

Store at -20°C
Shipped on dry ice