Bgl I

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Source

Bacillus globigii RUB 561

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl

Incubation Temperature

37°C

Heat Inactivation

65°C for 10 minutes

Reaction Buffer

50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
100 mM NaCl
1 mM dithiothreitol
100 μg/ml bovine serum albumin 

Storage Buffer

20 mM Tris‑HCl (pH 7.5 at 4°C)
200 mM NaCl
10 mM MgCl₂
1 mM dithiothreitol
0.1 mM EDTA
500 µg/ml bovine serum albumin
0.1% (v/v) Triton X-100™
50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 7.5 at 37°C)
100 mM NaCl
10 mM MgCl₂
1 mM dithiothreitol
100 μg/ml bovine serum albumin
1 μg lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 μl

Storage Conditions

Store at -20°C
Shipped on dry ice