BsiHKC I

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Source

Bacillus stearothermophilus HKC
Note: Purified from E coli strain that carries the cloned bsiHKCRI gene

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour in a total reaction volume of 50 μl

Incubation Temperature

65°C

Heat Inactivation

No

Reaction Buffer

10 mM Tris-HCl (pH 7.5 at 37°C)
50 mM NaCl
10 mM MgCl₂
1 mM DTT
100 µg/ml bovine serum albumin

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 4°C)
50 mM KCl
1 mM dithiothreitol
0.1 mM EDTA
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

10 mM Tris-HCl (pH 7.5 at 37°C)
50 mM NaCl
10 mM MgCl₂
1 mM DTT
100 µg/ml bovine serum albumin
1 µg lambda DNA

Storage Conditions

Store at -20°C
Shipped on dry ice