BstX I

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Source

Bacillus stearothermophilus X

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour at 55°C in a total reaction volume of 50 µl

Incubation Temperature

55°C

Heat Inactivation

65°C for 10 minutes

Reaction Buffer

50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
100 mM NaCl
1 mM DTT
100 µg/ml bovine serum albumin

Assay Conditions

50 mM Tris‑HCl (pH 7.5 at 37°C)
100 mM NaCl
10 mM MgCl₂
1 mM DTT
100 µg/ml BSA
1 µg lambda DNA

Incubation for 1 hour at 55°C in a total reaction volume of 50 µl

Storage Buffer

10 mM Tris‑HCl (pH 7.4 at 4°C)
50 mM KCl
0.1 mM EDTA
1 mM dithiothreitol
0.005% Triton X-100™
200 µg/ml bovine serum albumin
50% (v/v) glycerol

Storage Conditions

Store at -20°C
Shipped on dry ice