Cla I

Catalog No. 2110

Availability: In stock

Price From: $104.00

Quick Overview

5'...AT↓CGAT...3'
3'...TAGC↑TA...5'

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Price From: $104.00

Details

Source

Caryophanon latum L

Reagents Supplied

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

No

Incubation Temperature

37°C

Assay Unit Substrate

Lambda DNA

Heat Inactivation

65°C for 10 minutes

Note

Cla I is blocked by dam methylation

Reaction Buffer

CMX Buffer 4:
20 mM Tris-acetate  (pH 7.5 at 37°C)
50 mM potassium acetate
10 mM magnesium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin 

Activity in Buffers

Buffer 1: 75%
Buffer 2: 100%
Buffer 3: 100%
Buffer 4: 100%
Buffer 5: 100%

Storage Buffer

50 mM Tris-HCl (pH 7.4 at 4°C) 
0.1 mM EDTA 
200 mM KCl
5 mM β-mercaptoethanol
0.1% (v/v) Triton X-100™
500 µg/ml bovine serum albumin  
50% (v/v) glycerol

Assay Conditions

See Certificate of Analysis Below

Storage Conditions

Store at -20°C
Shipped on dry ice

Downloads

Certificate of Analysis
Lot 2102024 PDF 

MSDS
MSDS PDF