Hha I

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Source

Arthrobacter luteus

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl

Heat Inactivation

65°C for 10 minutes

Incubation Temperature

37°C

Reaction Buffer

10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Storage Buffer

20 mM Tris-HCl (pH 7.5 at 4°C)
100 mM KCl
0.5 mM DTT
500 µg/ml bovine serum albumin
0.1% (w/v) Triton X-100™
50% glycerol

Assay Conditions

10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
50 mM NaCl
1 mM dithiothreitol
1 µg lambda DNA

Storage Conditions

Store at -20°C
Shipped on dry ice