Hpa l

Catalog No. 2250

Availability: In stock

Price From: $50.00

Quick Overview

5'...GTT↓AAC...3'
3'...CAA↑TTG...5' 

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Price From: $50.00

Details

Source

Haemophilus parainfluenzae

Reagents Supplied 

CMX Buffer 4

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

No

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 4:
20 mM Tris-Acetate (pH 7.5 at 37°C)
50 mM potassium acetate
10 mM magnesium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 25%
Buffer 2: 50%
Buffer 3: 25%
Buffer 4: 100%
Buffer 5: 100%
*Star activity 

Storage Buffer

20 mM Tris-HCl (pH 7.4 at 4°C)
0.5 mM EDTA
50 mM KCl
1 mM DTT
0.1% (w/v) Triton X-100™
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

20 mM Tris‑Acetate (pH 7.5 at 37°C)
50 mM potassium acetate
10 mM magnesium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA

Storage Conditions

Store at -20°C
Shipped on dry ice 

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot