Details
Downloads
Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot
Source
Haemophilus parainfluenzae
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl
37°C
Heat Inactivation
65°C for 10 minutes
Reaction Buffer
10 mM Tris-HCl (pH 7.0 at 37°C)
10 mM MgCl2
1 mM dithiothreitol
100 µg/ml bovine serum albumin
Storage Buffer
20 mM Tris-HCl (pH 7.4 at 4°C)
0.5 mM EDTA
1 mM dithiothreitol
0.5 mg/ml bovine serum albumin
50% (v/v) glycerol
Assay Conditions
10 mM Tris-HCl (pH 7.0 at 37°C)
10 mM MgCl2
1 mM dithiothreitol
100 μg/ml bovine serum albumin
1 μg lambda DNA
Incubation is at 37°C for 1 hour in a reaction volume of 50 µl
Storage Conditions
Store at -20°C
Shipped on dry ice
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