Mbo ll

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Source

Moraxella bovis

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of unmethylated lambda DNA in 1 hour in a total reaction volume of 50 µl

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Reaction Buffer

50 mM Tris-HCl (pH 7.0 at 37°C)
10 mM MgCl₂100 mM NaCl
1 mM DTT
100 µg/ml bovine serum albumin

Storage Buffer

10 mM Tris-HCl (pH 7.5 at 4°C)
1 mM dithiothreitol
0.1 mM EDTA
50 mM KCl
0.01% Triton X-100™
100 ug/ml bovine serum albumin
50% glycerol

Assay Conditions

50 mM Tris‑HCl (pH 7.0 at 37°C)
10 mM MgCl₂
100 mM NaCl
1 mM DTT
100 µg/ml BSA
1 µg unmethylated lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice