Mlu I

Catalog No. 2287

Quick Overview

5'...A↓CGCGT...3'
3'...TGCGC↑A...5' 

* Required Fields

Price From: $54.00

Details

Source

Micrococcus luteus

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 25%
Buffer 2: 75%
Buffer 3: 100%
Buffer 4: 50%
Buffer 5: 50%
*Star activity 

Storage Buffer

50 mM Tris‑HCl (pH 7.4 at 4°C)
50 mM NaCl
0.1 mM EDTA
1 mM dithiothreitol
500 µg/ml bovine serum albumin
0.1 % Triton X-100™
50% (v/v) glycerol

Assay Conditions

50 mM Tris‑HCl (pH 7.5 at 37°C)
10 mM MgCl2
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 μg lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice 

Downloads

Certificate of Anaysis PDF -Current Lot
MSDS PDF -Current Lot

Categories

Restriction Endonucleases