Nco I

Catalog No. 2292

Quick Overview

5'...C↓CATGG...3'
3'...GGTAC↑C...5' 

* Required Fields

Price From: $52.00

Details

Nco I Source

Nocardia corallina

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3:
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 75%
Buffer 2: 75%
Buffer 3: 75%
Buffer 4: 100%
Buffer 5: 100%
*Star activity 

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)
100 mM KCl
1 mM EDTA
10 mM BME
500 µg/ml bovine serum albumin
0.1 mM PMSF
0.1% Triton X-100™
50% (v/v) glycerol

Assay Conditions

20 mM Tris Acetate (pH 7.5 at 37°C)
50 mM potassium acetate
10 mM magnesium acetate
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA

Storage Conditions

Store at -20°C
Shipped on dry ice 

Downloads

Certificate of Analysis PDF - Current Lot
MSDS PDF -Current Lot

Categories

Restriction Endonucleases