Nhe I

Catalog No. 2293

Quick Overview

5'...G↓CTAGC...3'
3'...CGATC↑G...5' 

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Price From: $32.00

Details

Nhe I Source

Neisseria mucosa heidelbergensis

Reagents Supplied

CMX Buffer 5

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Adenovirus 2 DNA

Reaction Buffer

CMX Buffer 5:
20 mM Tris-HCl (pH 7.0 at 37°C)
5 mM MgCl2
50 mM KCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 100%
Buffer 2: 100%
Buffer 3: 75%
Buffer 4: 75%
Buffer 5: 100%
*Star activity 

Storage Buffer

10 mM Tris-HCl (pH 7.4 at 4°C)
50 mM KCl
0.1 mM EDTA
0.1 mM dithiothreitol
0.1% (v/v) Triton X-100™
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions 

20 mM Tris-HCl (pH 7.0 at 37°C)
50 mM KCl
5 mM MgCl2 
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg Adenovirus 2 DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50µl

Quality Control

All preparations are assayed for contaminating endonuclease, 3'-exonuclease, and nonspecific single- and double-stranded DNase activities

Notes:

  1. Due to star activity, it is not recommended to incubate over 4 hours or to use more than 100 units per reaction.
  2. Salt concentration above 50 mM inhibits Nhe I activity.

Storage Conditions

Store at -20°C
Shipped on dry ice 

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Categories

Restriction Endonucleases