Nsi I

Catalog No. 2298

Availability: In stock

Price From: $55.00

Quick Overview

5'...ATGCA↓T...3'
3'...T↑ACGTA...5' 

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Price From: $55.00

Details

Nsi I Source

Neisseria sicca

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl

Star Activity

No

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 3
50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: <25%
Buffer 2: 75%
Buffer 3: 100%
Buffer 4: 75%
Buffer 5: 50%

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)
200 mM NaCl
0.1 mM EDTA
1 mM DTT
500 µg/ml bovine serum albumin
50% glycerol

Assay Conditions

50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg lambda DNA

Storage Conditions 

Store at -20°C
Shipped on dry ice 

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Categories

Restriction Endonucleases