Nsi I

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Source

Arthrobacter luteus

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl

Heat Inactivation

10 minutes at 65°C

Reaction Buffer

50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂ 
100 mM NaCl
1 mM DTT
100 µg/ml bovine serum albumin

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)
200 mM NaCl
0.1 mM EDTA
1 mM DTT
500 µg/ml bovine serum albumin
50% glycerol

Assay Conditions

50 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂ 
100 mM NaCl
1 mM DTT
100 µg/ml bovine serum albumin
1 µg lambda DNA

Storage Conditions 

Store at -20°C
Shipped on dry ice