PinA I

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Source

Pseudomonas inaequalis

Reagents Supplied

CMX Buffer 5

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in total reaction volume of 50 µl

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°

Reaction Buffer

20mM Tris-HCl (pH 7.0 at 37°C)
5 mM MgCl₂
50 mM KCl
1 mM dithiothreitol
0.1 mg/ml bovine serum albumin 

Storage Buffer

20 mM Tris-HCl (pH 7.5 at 4°C)
0.1 mM EDTA
100 mM NaCl
10 mM β-mercaptoethanol
0.05% (v/v) Triton X-100™
50% (v/v) glycerol

Assay Conditions

20 mM Tris‑HCl (pH 7.0 at 37°C)
5 mM MgCl₂
50 mM KCl
1 mM DTT
100 µg/ml bovine serum albumin
1 µg lambda DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice