Pvu I

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Source

Proteus vulgaris

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour at 37°C in a total reaction volume of 50 µl

Incubation Temperature

37°C

Heat Inactivation  

10 minutes at 80°C

Reaction Buffer

10 mM Tris-HCl (pH 8.0 at 37°C)
10 mM MgCl₂
150 mM KCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Storage Buffer

10mM Tris-HCl (pH 7.5 at 4°C)
50 mM KCl
0.1 mM EDTA
1 mM dithiothreitol
0.02% (v/v) Triton X-100™
150 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 8.0 at 37°C)
10 mM MgCl₂
50 mM NaCl
50 mM KCl
1 µg Adenovirus 2 DNA
Reaction volume 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice