Sac I

Catalog No. 2350

Availability: In stock

Price From: $50.00

Quick Overview

5'...GAGCT↓C...3'
3'...C↑TCGAG...5' 

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Price From: $50.00

Details

Sac I Source

Streptomyces achromogenes

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Adenovirus 2 DNA

Reaction Buffer

CMX Buffer 2;
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin - See more at: http://www.chimerx.com/store/restriction-endonucleases/hinfi#sthash.eTrsWtsh.dpuf

CMX Buffer 2:
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

CMX Buffer 2;
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin - See more at: http://www.chimerx.com/store/restriction-endonucleases/hinfi#sthash.eTrsWtsh.dpuf

Storage Buffer

20 mM Tris‑HCl (pH 8.0 at 4ºC)
150 mM KCl
500 µg/ml bovine serum albumin
0.1% Triton X-100™
50% (v/v) glycerol

Assay Conditions

10 mM Tris‑HCl (pH 7.5 at 37ºC)
10 mM MgCl2
1 mM dithiothreitol
1 µg Adenovirus 2 DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice 

Sac I Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Categories

Restriction endonucleases