Sac I

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Source

Streptomyces achromogenes

Reagents Supplied

CMX Buffer 1

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Reaction Buffer

10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl₂
1 mM dithiothreitol

Storage Buffer

20 mM Tris‑HCl (pH 8.0 at 4ºC)
150 mM KCl
500 µg/ml bovine serum albumin
0.1% Triton X-100™
50% (v/v) glycerol

Assay Conditions

10 mM Tris‑HCl (pH 7.5 at 37ºC)
10 mM MgCl₂
1 mM dithiothreitol
1 µg Adenovirus 2 DNA
Incubation is at 37°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice