SaI I

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot 

Source

Streptomyces albus

Unit Definition

One unit is the amount of enzyme required to completely digest 1 μg of Adenovirus 2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl

Incubation Temperature

37°C

Heat Inactivation 

10 minutes at 65°C

Reaction Buffer

50 mM Tris-HCl (pH 7.5 at 37ºC)
10 mM MgCl₂
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Storage Buffer

20 mM potassium phosphate (pH 7.0 at 4ºC) 
200 mM NaCl
1.0 mM EDTA
1 mM DTT
5 μg/ml Poly L-Lysine
0.0065% sodium azide
500 μg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 7.5 at 37ºC)
10 mM MgCl₂
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg adenovirus 2 DNA 

Storage Conditions

Store at -20°C
Shipped on dry ice