Sfi I

Catalog No. 2380

Quick Overview

5'...GGCCNNNN↓NGGCC...3'
3'...CCGGN↑NNNNCCGG...5' 

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Price From: $28.00

Details

Sfi I Source

Streptomyces fimbriatus

Reagents Supplied

CMX Buffer 2

Incubation Temperature

50°C

Heat Inactivation 

No

Assay Unit Substrate

Adenovirus 2 DNA

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Reaction Buffer

CMX Buffer 2:
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 25%
Buffer 2: 100%
Buffer 3: 25%
Buffer 4: 100%
Buffer 5: 50%
*Star activity 

Storage Buffer

5 mM potassium phosphate (pH 7.4 at 4°C)
300 mM NaCl
0.1 mM EDTA
1 mM dithiothreitol
0.15% Triton X-100™
100 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

10 mM Tris‑HCl (pH 7.5 at 37°C)
10 mM MgCl2
1 mM dithiothreitol
50 mM NaCl
1 µg of Adenovirus 2 DNA
100 µg/ml bovine serum albumin
Incubation is at 50°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice 

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Categories

Restriction Endonucleases