Spe I

Catalog No. 2398

Quick Overview

5'...A↓CTAGT...3'
3'...TGATC↑A...5' 

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Price From: $100.00

Details

Spe I Source

Sphaerotilus species

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Ad-2 DNA

Reaction Buffer

CMX Buffer 2:
10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 100%
Buffer 2: 100%
Buffer 3: 50%
Buffer 4: 75%
Buffer 5: 100%
*Star activity 

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C) 
200 mM NaCl 
0.1 mM EDTA
0.2% (v/v) Triton 
X-100™
1 mM dithiothreitol
500 µg/ml bovine serum albumin 
50% (v/v) glycerol

Assay Conditions

10 mM Tris-HCl (pH 7.5 at 37°C)
10 mM MgCl2
50 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
 1 µg Ad-2 DNA

Storage Conditions

Store at -20°C
Shipped on dry ice 

Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Categories

Restriction Endonucleases