Sph I

Catalog No. 2400

Quick Overview

5'...GCATG↓C...3'
3'...C↑GTACG...5' 

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Price From: $80.00

Details

Sph I Source

Streptomyces phaeochromogenes

Reagents Supplied

10X Sph I Reaction Buffer

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 μl

Star Activity

Yes

Reaction Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

10X Sph I Reaction Buffer:
50 mM Tris-HCl (pH 7.4 at Room Temperature)
6 mM MgCl2
50 mM NaCl
50 mM KCl

Activity in Buffers

Buffer 1: 25%
Buffer 2: 50%
Buffer 3: 75%
Buffer 4: 25%
Buffer 5: 25%
*Star activity 

Storage Buffer

50 mM Tris‑HCl (pH 7.5 at 4°C)
1 mM EDTA
100 mM KCl
5 mM β-mercaptoethanol
0.1% Triton X-100™
0.1 mM PMSF
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

50 mM Tris‑HCl (pH 7.4 at Room Temperature)
6 mM MgCl2
50 mM NaCl
50 mM KCl
1 µg lambda DNA

Storage Conditions

Store at -20°C
Shipped on dry ice 

Sph I Downloads

Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot

Categories

Restriction Endonucleases