Details
Downloads
Certificate of Analysis PDF -Current Lot
MSDS PDF -Current Lot
Source
Streptomyces phaeochromogenesUnit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 μlReaction Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Reaction Buffer
50 mM Tris-HCl (pH 7.4 at RT)
6 mM MgCl2
50 mM NaCl
50 mM KCl
Storage Buffer
50 mM Tris‑HCl (pH 7.5 at 4°C)
1 mM EDTA
100 mM KCl
5 mM β-mercaptoethanol
0.1% Triton X-100™
0.1 mM PMSF
500 µg/ml bovine serum albumin
50% (v/v) glycerol
Assay Conditions
50 mM Tris‑HCl (pH 7.4 at RT)
6 mM MgCl2
50 mM NaCl
50 mM KCl
1 µg lambda DNA
Storage Conditions
Store at -20°C
Shipped on dry ice
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