Taq I

Downloads

MSDS PDF

Source

Thermus aquaticus

Reagents Supplied

CMX Buffer 3

Unit Definition

One unit is the amount of enzyme required to completely digest ΦX-174 DNA in 1 hour in a total reaction volume of 50 μl

Reaction Temperature

65°C

Heat Inactivation

No

Reaction Buffer

50 mM Tris-HCI (pH 7.5 at 37°C)
10 mM MgCl₂
100 mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)
300 mM KCl
0.1 mM EDTA
10 mM β-mercaptoethanol
0.1% Triton X-100™
500 µg/ml bovine serum albumin
50% (v/v) glycerol

Assay Conditions

50 mM Tris‑HCl (pH 7.5 at 37°C)
10 mM MgCl₂
100mM NaCl
1 mM dithiothreitol
100 µg/ml bovine serum albumin
1 µg ΦX-174 DNA
Incubation is at 65°C for 1 hour in a total reaction volume of 50 µl

Storage Conditions

Store at -20°C
Shipped on dry ice